ViewSizer 3000

Simultaneous Multi-Laser Nanoparticle Tracking Analysis (NTA)

Exosomes? Virus? Nanoparticle? Use multiple lasers for complete, detailed analysis of all the particles in your sample.

Exosomes, viruses, and nanoparticles all have wide size distributions which defeat traditional Nanoparticle Tracking Analysis (NTA) analyzers. The ViewSizer 3000 features simultaneous measurement with three lasers to collect the most accurate distribution and concentration information over a wide range of sizes within the same sample. Where the signal from a particle is too bright and saturates the detector from one laser, the software automatically uses data from a lower power laser to ensure the most accurate size and concentration information. On the other hand, when scattering from one laser is too weak for detection, the software uses data from a higher power laser to accurately track the particle.

Cross contamination is a concern in all analyses. Simplified cleaning means thorough cleaning. The easy-to-remove sample cells can be dissassembled for rapid, thorough cleaning, which leads to better data.

Escape the limits of traditional NTA

Accurate and sensitive analysis without cross contamination

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Segment: Scientific

Division: Particle Characterization

Manufacturing Company: HORIBA Instruments Incorporated

The ViewSizer 3000 uses the latest advances in nanoparticle tracking analysis (NTA) to accurately determine particle properties!

Size Measurement Range: 10 nm to 15 microns depending on sample

The ViewSizer determines particle size and size distribution details by multi-laser nanoparticle tracking analysis (NTA). Multiple lasers enable analysis of a broad range of sizes in the same sample.

Concentration Measurement Range: 1E5 to 1E9 particles/mL

NTA can be used to count particles in the measurement volume. A patented method corrects for the effect of particle size on effective measurement volume.

No cross-contamination

The sample cell can be completely removed and dissassembled for complete cleaning. Disassembly, cleaning, and reassembly is faster than rinsing a flow cell. Furthermore, multiple cells can be used for faster throughput or assigned to various groups in a shared (core) facility.

Featured Publications for the ViewSizer 3000:

Biological characterization using protein crystal measurements	https://bioprocessintl.com/analytical/product-characterization/biological-characterization-using-protein-crystal-measurements/
A lipase-independent pathway of lipid release and immune modulation by adipocytes	https://science.sciencemag.org/content/363/6430/989
Application of a novel new multispectral nanoparticle tracking technique	https://iopscience.iop.org/article/10.1088/1361-6501/aab940/meta
Biophysical characterization of polydisperse liposomal adjuvant formulations	https://doi.org/10.1016/j.bbrc.2020.05.156
Characterisation of particles in solution – a perspective on light scattering and comparative technologies	https://doi.org/10.1080/14686996.2018.1517587
Cyclodextrin Reduces Intravenous Toxicity of a Model Compound	https://doi.org/10.1016/j.xphs.2019.01.004
Development and anti-Candida evaluation of the vaginal delivery system of ... nanosuspension-loaded thermogel	https://doi.org/10.1080/1061186X.2018.1434660
Electrochemical sensor based on F,N-doped carbon dots decorated laccase for detection of catechol	https://doi.org/10.1016/j.jelechem.2019.03.071
Light scattering by pure water and seawater: the depolarization ratio and its variation with salinity	https://doi.org/10.1364/AO.58.000991
Lipid Nanoparticle-Delivered Chemically Modified mRNA Restores Chloride Secretion in Cystic Fibrosis	https://doi.org/10.1016/j.ymthe.2018.05.014
Mesenchymal Stromal Cell Bioreactor for Ex Vivo Reprogramming of Human Immune Cells	https://doi.org/10.1038/s41598-020-67039-w
Multifunctional Nanocomposites Based on Liposomes and Layered Double Hydroxides Conjugated with Glycylsarcosine for Efficient Topical Drug Delivery to the Posterior Segment of the Eye	https://doi.org/10.1021/acs.molpharmaceut.8b01136
Particle size analysis of polydisperse liposome formulations with a novel multispectral advanced nanoparticle tracking technology	https://doi.org/10.1016/j.ijpharm.2019.06.013
Review of nanoparticles in ultrapure water: definitions and current metrologies for detection and control	https://www.ovivowater.com/review-of-nanoparticles-in-ultrapure-water-definitions-and-current-metrologies-for-detection-and-control/
Spark erosion as a high-throughput method for producing bimodal nanostructured 316L stainless steel powder	https://doi.org/10.1016/j.powtec.2018.01.012
Synthesis and Characterization of EGFR-Targeted Immunoporphysomes	http://hdl.handle.net/1807/89548
Synthesis of Ultrasmall Synthetic Melanin Nanoparticles by UV Irradiation in Acidic and Neutral Conditions	https://pubs.acs.org/doi/abs/10.1021/acsabm.9b00747
Nanoparticle Tracking Analysis for the Quantification and Size Determination of Extracellular Vesicles \| Protocol (jove.com)	https://doi.org/10.3791/62447
Isolation and characterization of EV in Saliva of Children with Asthma	https://evcna.com/article/view/3962
Spinal cord injury alters microRNA and CD81+ exosome levels in plasma extracellular nanoparticles with neuroinflammatory potential	https://doi.org/10.1016/j.bbi.2020.12.007
Skeletal muscle tissue secretes more extracellular vesicles than white adipose tissue and myofibers are a major source ex vivo but not in vivo	https://doi.org/10.1101/2020.09.27.313932
Human milk extracellular vesicle miRNA expression and associations with maternal characteristics in a population-based cohort from the Faroe Islands	https://www.nature.com/articles/s41598-021-84809-2
Purification of Cas9 - RNA Complexes by Ultrafiltration	https://doi.org/10.1002/btpr.3104
Assessment of Surface Glycan Diversity on Extracellular Vesicles by Lectin Microarray and Glycoengineering Strategies for Drug Delivery Applications	https://doi.org/10.1002/smtd.202100785
Bioactive extracellular vesicles from a subset of endothelial progenitor cells rescue retinal ischemia and neurodegeneration	https://doi.org/10.1172/jci.insight.155928
Circulating Extracellular Vesicles From Septic Mice Induce Brain Inflammation via Mirna Cargo	http://hdl.handle.net/10713/19055

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Measurement Range	10 nm to 15 μm
Typical Sample Volume	350 µL to 2.5 mL
Typical Sample Concentration (Sample dependent)	10^5 – 10^9 particles/mL
Sample Temperature Range (Controlled)	10° C to 50° C +/- 0.1° C
Dimensions	55 cm W x 66 cm D x 35 cm H
Weight	27 kg
Operational Environment	15° C to 30° C with < 85% RH

Particle Analysis in Vaccine Manufacturing and Development

Size matters in vaccine delivery systems. Nanoparticles smaller than 200 nm generally present a greater immunogenic response than micro-particles larger than 1 micron.

Achieving Infectious Titer with Multi-laser Nanoparticle Tracking Analysis (NTA)

In this note, the upstream process of analyzing viral preparations is addressed, as well as the use of multi-laser nanoparticle tracking analysis (NTA) as a cost and time efficient method to measure size, count virus particles, and correlate to infectious titer.

Optimization of an Emulsion Polymerization Process and Product Through Nanoparticle Concentration Analysis

This note covers the history, theory and processes used for emulsion polymerization. It also examines the importance of measuring the concentration of latex nanoparticles produced by emulsion polymerization for a biotech application developed by the Gianneschi lab at UCSD.

Predicting Whiskey Shelf Stability with Particle Size Distribution

Careful control of the particulates in whiskey is an important step to quality flavor and color. Inadequate monitoring of particle size impedes the final product stability, consistency, quality, and price. In this note, the particle science behind these determining factors will be thoroughly examined and explained.

Optimization of High-Performance Nanostructured Powder Metallurgy Materials

In this note, we will briefly explore the history of powder metallurgy and then examine the importance of powder quality to the production of nanomaterials.

Nanobubble Size and Concentration Analysis with Multi-laser NTA

This note analyzes carbon dioxide and oxygen gas nanobubbles used for pain management to demonstrate multi-laser Nanoparticle Tracking Analysis’s ability to characterize a mixture of nano and microbubbles without sample manipulation.

Protein aggregation is an important concern when developing and manufacturing biotherapeutics. It is important to quantify protein aggregation and particle formulation as this data is needed to guide formulation development, evaluate handling requirements, and monitor product quality.

Size Distribution and Concentration of Extracellular Vesicles

In this study, we demonstrate the ViewSizer 3000’s capabilities as a next generation NTA based analysis instrument in order to accurately and efficiently measure and characterize extracellular vesicles (EV) via particle size and concentration.

Characterization of Sub-Visible Particles in Protein Therapeutic Formulations

The ViewSizer™ 3000 enables scientists to visualize and quantify the kinetics of protein aggregation in real-time for biologic therapeutics under a variety of stress conditions.

Virtually any metal can be made into a powder through processes including atomization, electrolysis, chemical reduction, and size comminution. The metal powder can then be used to fabricate parts from simple to complex shape. The technique and study of these processes is known as powder metallurgy, a field of study well acquainted with particle characterization. The size distribution and shape of the powder metal particles influences the physical properties of the parts created.

Quantifying Trace Amount of Nanoparticles in Drinking Water

The concern of water contamination from nanoplastics (≤ 1 µm) is a study receiving close scrutiny from FDA and EPA alike. Nanoparticles can gain access through inhalation, ingestion, or dermal exposure and have greater cellular uptake than those bigger in size. Nanoparticles subsequently pose a greater impact on health.

A virus particle consists of several parts including the genetic material (DNA or RNA), a protein coat that protects these genes, and in some cases an envelope of lipids that surrounds the protein coat when they are outside a cell.

Enhanced Characterization of Fluorescently Labeled Extracellular Vesicles using ViewSizer 3000

Exosomes are small extracellular vesicles, 30-150 nm in diameter, which have been determined to play a crucial role in extracellular signaling. They have been observed in both prokaryotic and eukaryotic organisms, meaning they are incredibly widely spread in nature. Exosomes bud off from their parent cells in a sealed package, taking the properties of their parent cell walls with them and encasing many intracellular components.

Comparison of Extracellular Vesicle Purification Methods

Extracellular vesicles (EVs) are particles secreted by cells and contain valuable information for both diagnostic and therapeutic purposes. However, the collection, storage, preparation, and separation of samples significantly influence EV yield both quantitatively and qualitatively.

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Particle Guidebook